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===== Check the filter parameters used in identification results ===== | ===== Check the filter parameters used in identification results ===== | ||
- | [[how_to:idffilters|How to check filter parameters used in identification results]]\\ | + | A set of properties are accessible for each identification and can be [[how_to:accessproperties|displayed or used to filter the list]]. |
- | [[how_to:accessproperties|how to filter columns in a tree view]] | + | This is specifically useful to [[how_to:idffilters|check that same filter parameters]] were used for all identification results validation.\\ |
===== Structure your experiment as a context tree ===== | ===== Structure your experiment as a context tree ===== | ||
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===== Execute protein grouping for every contexts ===== | ===== Execute protein grouping for every contexts ===== | ||
{{:usecase1_groupedcontexthierarchy.png |}} | {{:usecase1_groupedcontexthierarchy.png |}} | ||
- | A context compile a number of identifications results. A same protein may be present in several identifications. So, there is a need to suppress this redundancy by [[userguide:peptidesandproteingrouping|grouping proteins]] at the context level. [[how_to:proteingrouping|Execute the protein grouping algorithm]] for all the User contexts in a specific order.\\ | + | Contexts representing preparation protocol compile a number of identifications results. A same protein may be present in several identifications. So, the first step is to suppress this redundancy by [[userguide:peptidesandproteingrouping|grouping proteins]] at the context level. [[how_to:proteingrouping|Execute the protein grouping algorithm]] for all the User contexts in this order:\\ |
* Start with the contexts that directly group the identification results: "Ultrafiltration", "Proteominer" and "TCA Precipitation". Do that for the two replicates. | * Start with the contexts that directly group the identification results: "Ultrafiltration", "Proteominer" and "TCA Precipitation". Do that for the two replicates. | ||
* Then continue with the contexts "Replicate1" and "Replicate2". | * Then continue with the contexts "Replicate1" and "Replicate2". | ||
* And finally, end with the root context named "ProjectABC" | * And finally, end with the root context named "ProjectABC" | ||
\\ | \\ | ||
+ | |||
+ | |||
If everything has worked correctly, all the context names are now in red. | If everything has worked correctly, all the context names are now in red. | ||
===== Browse the context tree and export it ===== | ===== Browse the context tree and export it ===== | ||
- | [[how_to:browsecontextprotgroups|How to browse a context]]. | + | [[how_to:browsecontextprotgroups|How to browse a context]].\\ |
+ | [[how_to:exportcontext|Export identified proteins from a context]]. | ||
===== Check reproducibility of results ===== | ===== Check reproducibility of results ===== | ||
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FIXME a deplacer FIXME\\ | FIXME a deplacer FIXME\\ | ||
[[how to:comparetwocontexts|How to compare contexts each other]].\\ | [[how to:comparetwocontexts|How to compare contexts each other]].\\ | ||
- | See the following link to understand [[userguide:contextcomparisons|what is context comparison]]. | + | See the following link to understand [[userguide:contextcomparisons|what is context comparison]].\\ |
+ | [[how_to:exportcomparison|Export comparison results]].\\ | ||
===== Find the optimal preparation protocol ===== | ===== Find the optimal preparation protocol ===== | ||
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===== Compare a parent context with their child contexts and export comparison results ===== | ===== Compare a parent context with their child contexts and export comparison results ===== | ||
FIXME a deplacer FIXME | FIXME a deplacer FIXME | ||
- | * [[how_to:comparemultiplecontexts|How to compare a context with its children]]. | + | [[how_to:comparemultiplecontexts|How to compare a context with its children]].\\ |
- | * Show covering diagram | + | Show covering diagram\\ |
- | * Export results | + | [[how_to:exportcomparison|Export comparison results]].\\ |
See the following link to understand [[userguide:contextcomparisons|what is context comparison]]. | See the following link to understand [[userguide:contextcomparisons|what is context comparison]]. | ||
===== Save the context hierarchy ===== | ===== Save the context hierarchy ===== | ||
- | | + | [[how_to:savetomsidb|Save back to the MSIdb]].\\ |