Once a result file have been imported and a search result created, the validation is performed in 4 mains steps :

1. Peptide Matches filtering and validation
2. Peptide Matches filtering and Validation
3. Protein Inference (peptides and proteins grouping)
4. Protein and Proteins Sets scoring
5. Protein Sets Filtering and Validation

Finally, the Identification Result issued from these steps is stored in the identification database. Different validation of a Search Result can be performed and a new Identification Summary of this Search Result is created for each validation.