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Table of Contents
Search Result and Identification Summary
A Search Result (aka ResultSet in the database schema) is the raw interpretation of a given set of MS/MS spectra given by a search engine or a denovo interpretation process. The content of a Search Result is one or many peptides matching to the submitted MSMS spectrum (PSM), each peptide being issued from protein sequences. The Search Result also contains additional information such as search parameters, used data-bank, etc. A Search Result is created when a resultfile (Mascot .dat file or an OMSSA .omx) file is imported in Proline, in the case of a target-decoy search, two Search Results are created : one for the target PSM, one for decoy PSM.
An Identification Summary (aka ResultSummary) is a set of identified proteins inferred from a subset of the PSM contained in the *Search Result*. The subset of PSM taken into account are the PSM that have been validated a filtering process (example: PSM fulfilling some specified criteria such as score greater than a threshold value).
Content of a Search Result
Importing a resultfile creates a new Search Result in the database which contains the following informations:
- Search Settings : software name and version, parameters values
- Peak List and Spectrum information : file name, ms-level, precursor moz, …
- Search result data:
- Proteins
- Peptide
- Spectrum
- 2 type of Matches
- a Peptide Match is a match between a peptide and a spectrum, with score, fragment matches …
- a Protein Matches is a match between a peptide and a protein
Mascot result importation
: todo
The peptide matches score correspond to Mascot ion score
OMSSA result importation
: todo
Content of an Identification Summary
- Protein Set :
- Peptide Set :
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