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prolineconcepts:rs

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Search Result

A Search Result (aka ResultSet in the database schema) is the raw interpretation of a given set of MS/MS spectra given by a search engine or a de novo interpretation process. It contains one or many peptides matching the submitted MS/MS spectra (PSM, i.e. Peptide Spectrum Match), and the protein sequences these peptides belong to. The Search Result also contains additional information such as search parameters, used protein sequence data-bank, etc.

A Search Result is created when a resultfile (Mascot .dat file or an OMSSA .omx) file is imported in Proline. In the case of a target-decoy search, two Search Results are created: one for the target PSMs, one for decoy PSMs.

Content of a Search Result

Importing a resultfile creates a new Search Result in the database which contains the following information:

  • Search Settings: software name and version, parameters values
  • Peak-List and Spectrum information: file name, MS level, precursor m/z, …
  • Search result data:
    • Protein sequences
    • Peptide sequences
    • Spectra
    • 2 kinds of Matches
      • Peptide Spectrum Matches, i.e. the matching between a peptide and a spectrum, with some related data such as the score, fragment matches…
      • Protein Matches, i.e. the proteins in the databank corresponding to the PSMs identified by the search engine

Mascot result importation

FIXME : todo : Mascot specificities

The peptide matches score correspond to Mascot ion score

OMSSA result importation

FIXME : todo : OMSSA specificities

The peptide matches score correspond to the negative common logarithm of the E-value:

  • Score = -log10(E-value)
prolineconcepts/rs.1411977844.txt.gz · Last modified: 2014/09/29 10:04 by 130.79.67.121