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prolineconcepts:rs

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Search Result

A Search Result (aka ResultSet in the database schema) is the raw interpretation of a given set of MS/MS spectra given by a search engine or a de novo interpretation process. It contains one or many peptides matching the submitted MS/MS spectra (PSM, i.e. Peptide Spectrum Match), and the protein sequences these peptides belong to. The Search Result also contains additional information such as search parameters, used protein sequence data-bank, etc.

A Search Result is created when a resultfile (Mascot .dat file or an OMSSA .omx) file is imported in Proline. In the case of a target-decoy search, two Search Results are created: one for the target PSMs, one for decoy PSMs.

Content of a Search Result

Importing a resultfile creates a new Search Result in the database which contains the following informations:

  • Search Settings : software name and version, parameters values
  • Peak List and Spectrum information : file name, ms-level, precursor moz, …
  • Search result data:
    • Proteins
    • Peptide
    • Spectrum
    • 2 type of Matches
      • a Peptide Match is a match between a peptide and a spectrum, with score, fragment matches …
      • a Protein Matches is a match between a peptide and a protein

Mascot result importation

FIXME : todo : Mascot specificities

The peptide matches score correspond to Mascot ion score

OMSSA result importation

FIXME : todo : OMSSA specificities

prolineconcepts/rs.1383930305.txt.gz · Last modified: 2013/11/08 18:05 by 193.48.0.3