In order to create and run Quantitation analyses, you must register your RAW files and corresponding MzDB files into Proline databses To do so, click on the “Settings” button in the top bar of the Dataset Explorer application, and go to the “Raw File Registerer” tab.

• In the left grid, use the “Add Raw Files to Selection” button in order to select Raw Files
• In the right grid, use the “Add MzDB Files to Selection” button in order to select MzDB Files
• You can then make automatic pairs, based on file names, by clicking on the “Make Couples from RAW Files” button. This will automatically add matching files into the “Raw & MzDB Couples” grid.

If you want to pair files whose names differ, you can proceed as follows :

• Select a file, either Raw or MzDB, in its grid,
• Click on the “Put in Couple” button,
• Select it in the “Raw & MzDB Couples” grid,
• Select the corresponding file of the other format (RAW or MzDB) in its grid,
• Click on its “Put in Couple” button.

After this, you must choose an Instrument Name and select the owner of the file in the users list.

Finally, click on “Register”. Note that this operation will fail if one of your raw file has already been registered.

Close the Settings Window.