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Once your Result Files have been imported, you can use them to create a new Identification Dataset. To create it from result files, you can:
Note that you can also create an empty dataset to further assemble complex structures using drag and drop in the project tree. However, this way is not favoured.
You should now see a window asking to choose a source of data for your new Dataset.
The only option available yet is Result Set: it allows you to build a new dataset from the Result Files you have imported.
Click OK to continue.
In the right panel, two text fields allow you to enter a name and description (optional) for your dataset.
By default, the result sets will be named in the tree using the result file name. You can change that with the Name child results using box. The possibilities are: peaklist file name, raw file identifier, result file name, sample, search title.
To add one or many files to your selection, select them in the grid (you can use the Ctrl and the Shift keys to make a multiple selection), then click on Add to dataset (top-right of the panel). You can also double click on one file to quickly add it to the selection.
To remove any file from the selection, just select them and click on Remove selected Items.
The creation of your identification dataset happens as follows:
Once your Identification Dataset has been created, you can see it on the tree, in the left side of the window. The white icon let you known that it is not yet validated (becomes green when validated).
Double click on the aggregation node to open the identification summary. This panel shows a list of your Identification fractions (corresponding to each imported file) and, after the validation process, it will display the Merged Result Summary infos.
Refer to the previous paragraph to know how to do these steps.
Once you have selected your result files, click on Add annotations. A window shows up, with as many empty lines as selected files.
Let's say you compare 2 conditions, and you have the following result table (Excel file for instance):
Result file | Peaklist File | Condition |
F078594.dat | OVEMB150205_21.raw.mgf | UPS1 50fmol |
F078596.dat | OVEMB150205_23.raw.mgf | UPS1 50fmol |
F078592.dat | OVEMB150205_25.raw.mgf | UPS1 50fmol |
F078590.dat | OVEMB150205_27.raw.mgf | UPS1 50fmol |
F078591.dat | OVEMB150205_12.raw.mgf | UPS1 5fmol |
F078595.dat | OVEMB150205_14.raw.mgf | UPS1 5fmol |
F078597.dat | OVEMB150205_16.raw.mgf | UPS1 5fmol |
F078593.dat | OVEMB150205_18.raw.mgf | UPS1 5fmol |