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prolineconcepts:decoysearches [2015/07/10 15:18] 132.168.72.225 |
prolineconcepts:decoysearches [2016/07/07 14:20] (current) 132.168.72.225 |
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| Proline handles decoy searches performed from two different strategies: | Proline handles decoy searches performed from two different strategies: | ||
| * Concatenated searches: | * Concatenated searches: | ||
| - | * A protein databank is created by concatenating target protein sequence to decoy protein sequence. Deoy could be created using reverse or random strategy. From Mascot or OMSSA point of view, a unique search is done using that databank. | + | * A protein databank is created by concatenating target protein sequence to decoy protein sequence. Decoy could be created using reverse or random strategy. From Mascot or OMSSA point of view, a unique search is done using that databank. |
| * Separated searches: | * Separated searches: | ||
| * Two searches are done using the same peaklist, one on a target protein databank and one on a decoy protein databank. These searches are then combined to retrieve useful information such as FDR ... Mascot allows user to check a decoy option and will automatically create a decoy databank. | * Two searches are done using the same peaklist, one on a target protein databank and one on a decoy protein databank. These searches are then combined to retrieve useful information such as FDR ... Mascot allows user to check a decoy option and will automatically create a decoy databank. | ||