prolineconcepts:decoysearches
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prolineconcepts:decoysearches [2013/11/21 14:12] 132.168.72.225 |
prolineconcepts:decoysearches [2016/07/07 14:20] (current) 132.168.72.225 |
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| Proline handles decoy searches performed from two different strategies: | Proline handles decoy searches performed from two different strategies: | ||
| * Concatenated searches: | * Concatenated searches: | ||
| - | * A protein databank is created by concatenating target protein sequence to decoy protein sequence. Deoy could be created using reverse or random strategie. From Mascot or OMSSA point of view, a unique search is done using that databank. | + | * A protein databank is created by concatenating target protein sequence to decoy protein sequence. Decoy could be created using reverse or random strategy. From Mascot or OMSSA point of view, a unique search is done using that databank. |
| * Separated searches: | * Separated searches: | ||
| - | * Two searches are done using the same peaklist, one on a target protein databank and one on a decoy protein databank. These searches are then combinated to retrieve usefull information such as FDR ... Mascot allows user to check a decoy option and will automalically create a decoy databank. | + | * Two searches are done using the same peaklist, one on a target protein databank and one on a decoy protein databank. These searches are then combined to retrieve useful information such as FDR ... Mascot allows user to check a decoy option and will automatically create a decoy databank. |
| Decoy and Target Search Result | Decoy and Target Search Result | ||
| * Concatenated searches: | * Concatenated searches: | ||
| - | * When importing search result from a decoy concatenated databank, decoy data are extracted from the Result File and stored in Proline databases as an independant/unique Search Result as well as target Search result data. These both searches are linked to each other. | + | * When importing search result from a decoy concatenated databank, decoy data are extracted from the Result File and stored in Proline databases as an independent/unique Search Result as well as target Search result data. These both searches are linked to each other. |
| * Separated searches | * Separated searches | ||
| * The two performed searches are stored in Proline databases and are linked together. | * The two performed searches are stored in Proline databases and are linked together. | ||
| See [[prolineconcepts:rs|Search Result]] to view which information is saved | See [[prolineconcepts:rs|Search Result]] to view which information is saved | ||
prolineconcepts/decoysearches.1385039557.txt.gz · Last modified: 2013/11/21 14:12 by 132.168.72.225
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