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prolineconcepts:decoysearches

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prolineconcepts:decoysearches [2013/09/26 10:15]
132.168.72.53 créée
prolineconcepts:decoysearches [2016/07/07 14:20] (current)
132.168.72.225
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 Proline handles decoy searches performed from two different strategies: Proline handles decoy searches performed from two different strategies:
   * Concatenated searches:   * Concatenated searches:
 +    * A protein databank is created by concatenating target protein sequence to decoy protein sequence. Decoy could be created using reverse or random strategy. From Mascot or OMSSA point of view, a unique search is done using that databank. ​
   * Separated searches: ​   * Separated searches: ​
 +    * Two searches are done using the same peaklist, one on a target protein databank and one on a decoy protein databank. These searches are then combined to retrieve useful information such as FDR ... Mascot allows user to check a decoy option and will automatically create a decoy databank. ​
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 +Decoy and Target Search Result
 +  * Concatenated searches:
 +    * When importing search result from a decoy concatenated databank, decoy data are extracted from the Result File and stored in Proline databases as an independent/​unique Search Result as well as target Search result data. These both searches are linked to each other.
 +  * Separated searches
 +    *  The two performed searches are stored in Proline databases and are linked together.
 +
 +See [[prolineconcepts:​rs|Search Result]] to view which information is saved
prolineconcepts/decoysearches.1380183308.txt.gz · Last modified: 2013/09/26 10:15 by 132.168.72.53