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prolineconcepts:decoysearches [2013/09/26 10:15] 132.168.72.53 créée |
prolineconcepts:decoysearches [2016/07/07 14:20] (current) 132.168.72.225 |
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Proline handles decoy searches performed from two different strategies: | Proline handles decoy searches performed from two different strategies: | ||
* Concatenated searches: | * Concatenated searches: | ||
+ | * A protein databank is created by concatenating target protein sequence to decoy protein sequence. Decoy could be created using reverse or random strategy. From Mascot or OMSSA point of view, a unique search is done using that databank. | ||
* Separated searches: | * Separated searches: | ||
+ | * Two searches are done using the same peaklist, one on a target protein databank and one on a decoy protein databank. These searches are then combined to retrieve useful information such as FDR ... Mascot allows user to check a decoy option and will automatically create a decoy databank. | ||
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+ | Decoy and Target Search Result | ||
+ | * Concatenated searches: | ||
+ | * When importing search result from a decoy concatenated databank, decoy data are extracted from the Result File and stored in Proline databases as an independent/unique Search Result as well as target Search result data. These both searches are linked to each other. | ||
+ | * Separated searches | ||
+ | * The two performed searches are stored in Proline databases and are linked together. | ||
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+ | See [[prolineconcepts:rs|Search Result]] to view which information is saved |