mzdb_documentation
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mzdb_documentation [2013/04/05 10:04] 193.48.0.3 créée |
mzdb_documentation [2016/01/14 16:09] (current) 193.48.0.3 |
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| - | ==== Installation procedure ==== | + | ==== raw2mzDB installation ==== |
| - | - get the zip archive | + | - Install MSFileReader from Thermo (see below) |
| - | - installation of MSFileReader from Thermo ( [[http://sjsupport.thermofinnigan.com/public/detail.asp?id=703|here]], will install all necessary c++ redistribuables) | + | |
| - Ensure your regional settings parameters are '.' for the decimal symbol and ',' for the list separator | - Ensure your regional settings parameters are '.' for the decimal symbol and ',' for the list separator | ||
| + | - Download the [[http://proline.profiproteomics.fr/wp-content/uploads/sites/2/2015/02/raw2mzdb_x64_0.9.7.zip|zip archive]] and unzip it | ||
| + | - See last section of this page to know how to use the tool | ||
| - | ==== Use case procedure ==== | + | ==== MSFileReader 3.0 installation (required by raw2mzDB and ProteoWizard) ==== |
| + | |||
| + | MSFileReader is the standard library used by raw2mzDB to access Thermo raw files. | ||
| + | |||
| + | - Create a Thermo account at https://thermo.flexnetoperations.com/control/thmo/RegisterMemberToAccount | ||
| + | - Once you are registered, you can download MSFileReader 3.0 from https://thermo.flexnetoperations.com/control/thmo/login (you will need to enter your login information) | ||
| + | - Go to the “Utility Software” section to download the file | ||
| + | - Unzip it, right click on the executable and left click on “Run as Administrator” | ||
| + | - Install it with defaults settings | ||
| + | - Install both 32-bit and 64-bit versions if possible (it will install all necessary c++ redistribuables) | ||
| + | |||
| + | ==== How to use the tool ? ==== | ||
| Open a command line window in the directory containing raw2mzdb.exe\\ | Open a command line window in the directory containing raw2mzdb.exe\\ | ||
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| There are two other available conversion modes: | There are two other available conversion modes: | ||
| - | - "profile", the command line is then: **raw2mzdb.exe -i <rawfilename> -o <outputfilename> -p 1** (means you want profile mode for MS1, others MSs will be stored as they were stored in the raw file) | + | - "profile", the command line is then: **raw2mzdb.exe -i <rawfilename> -o <outputfilename> -p 1** (means you want profile mode for MS1, others MS will be stored as they were stored in the raw file) |
| - | - "centroid" : **raw2mzdb.exe -i <rawfilename> -o <outputfilename> -c 1** (means you want centroid mode for MS1, others MSs will be stored as they were stored in the raw file) | + | - "centroid" : **raw2mzdb.exe -i <rawfilename> -o <outputfilename> -c 1** (means you want centroid mode for MS1, others MS will be stored as they were stored in the raw file) |
mzdb_documentation.1365149069.txt.gz · Last modified: 2013/04/05 10:04 by 193.48.0.3
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