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how_to:web:rsvalidation [2013/08/29 10:08] 193.48.0.3 |
how_to:web:rsvalidation [2016/07/06 17:10] (current) 193.48.0.3 |
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- | To launch a validation on a dataset, click on its node in the Project Tree on the left side of the Dataset Explorer. | + | ====== Validate a Search Result ====== |
- | {{:how_to:web:identification_overview.png?800|}} | ||
- | Click on “Launch Validation” in the toolbar of the Infos tab. | + | To launch a validation on a dataset: |
+ | * right-click on its node in the project tree then select //Validate tree// or | ||
+ | * double-click to open identification table, then click //Validate tree// in the toolbar. | ||
- | You can also right click on the dataset node and click on use the "Launch Validation" button. | + | {{ :how_to:web:dse_validate_tree_button.png?nolink&1000 |}} |
The following form appears : | The following form appears : | ||
- | {{:how_to:web:validation_form.png?800|}} | + | {{ :how_to:web:dse_validate_tree_params.png?nolink&800 |}} |
- | The Validation form handles several settings : | + | The validation form handles several settings, especially : |
- | - Merge Result Sets : choosing “YES” will merge the Result Sets (corresponding to your result files) before launching the validation of the merged dataset. “NO” will validate the result sets separately and merge their result after the validation process. | + | * PSM Filters : use the selection box and the green button to add more filters on Peptide Spectrum Matches to be applied during the validation process : Score, Rank, Sequence Length, E-Value and P-Value. |
+ | * PSM Validation Parameters : choose on which param the Peptide FDR should be applied : Score, E-Value or P-Value. | ||
- | - Filters : let you filter the data that will be included in the validation results. For example, you might want to keep only the peptide of rank 1. To add a filter parameter, choose a setting in the selection box, click on the “+” button, then edit the threshold value of the parameter on its line. | + | * Merging Mode : determines if the merging operation should be done before the validation (on result sets) or after the validation process (on identification summaries) |
- | + | ||
- | - Validation Thresholds let you define the False Discovery Rate of your peptides and proteins. You must define from which parameter the Peptide FDR should be estimated : FIXME (MORE DETAILS) | + | |
- | + | ||
- | When ready, click on “Validate” to launch the validation task. You can see it in the tasks panel : | + | |
- | + | ||
- | {{:how_to:web:validation_launched.png?600|}} | + | |
+ | When ready, click on //Validate// to launch the validation task. You can see it in the tasks panel. | ||
+ | When the validation finishes, a notification will be displayed and the node icon will be colored in green. | ||
+ | See [[prolineconcepts:rsvalidation| more details about the validation process]]. | ||