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how_to:studio:viewprotset [2014/09/09 15:10] 132.168.72.206 |
how_to:studio:viewprotset [2016/11/10 15:17] (current) 130.79.67.200 [PTM Protein Sites Window] |
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| - | ====== Display PSM, Peptides or Protein Sets of an Identification Summary ====== | + | ====== Display MS Queries, PSM, Peptides, Protein Sets, PTM Protein Sites or Adjacency Matrices ====== |
| - | ===== Functionnality Access ===== | + | ===== Functionality Access ===== |
| To display data of an Identification Summary: | To display data of an Identification Summary: | ||
| Line 8: | Line 8: | ||
| - right click on an Identification Summary | - right click on an Identification Summary | ||
| - | - click on the menu "Identification Summary >" and on the sub-menu "PSM", "Peptides" or "Protein Sets" | + | - click on the menu "Display Identification Summary >" and on the sub-menu "MSQueries", "PSM", "Peptides", "Protein Sets", "PTM Protein Sites" or "Adjacency Matrices" |
| - | {{:how_to:studio:displayidspopupv3.png|}} | + | {{:how_to:studio:popupdisplayidentification.png|}} |
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| + | ===== MSQueries Window ===== | ||
| + | |||
| + | If you click on MSQueries sub-menu, you obtain this window: | ||
| + | |||
| + | {{:how_to:studio:msqueryrsm.png|}} | ||
| + | |||
| + | Note: Abbreviations used are listed [[how_to:studio:abbreviations|here]] | ||
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| {{:how_to:studio:displayidspsmdialogv3.png|}} | {{:how_to:studio:displayidspsmdialogv3.png|}} | ||
| - | Note : Abbreviations used are listed [[how_to:studio:abbreviations|here]] | + | Note: Abbreviations used are listed [[how_to:studio:abbreviations|here]] |
| ===== Peptides Window ===== | ===== Peptides Window ===== | ||
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| - | **Upper View** : list of all Peptides | + | **Upper View**: list of all Peptides |
| - | **Middle View** : list of all Protein Sets containing the selected peptide. | + | **Middle View**: list of all Protein Sets containing the selected peptide. |
| - | **Bottom Left View** : list of all Proteins of the selected Protein Set | + | **Bottom Left View**: list of all Proteins of the selected Protein Set |
| - | **Bottom Right View** : list of all Peptides of the selected Protein | + | **Bottom Right View**: list of all Peptides of the selected Protein |
| - | Note : Abbreviations used are listed [[how_to:studio:abbreviations|here]] | + | Note: Abbreviations used are listed [[how_to:studio:abbreviations|here]] |
| ===== Protein Sets Window ===== | ===== Protein Sets Window ===== | ||
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| - | **View 1** (at the top) : list of all Protein Sets | + | **View 1** (at the top): list of all Protein Sets |
| + | |||
| + | Note : In the column Proteins, 8 (2, 6) means that there are 8 proteins in the Protein Set. 2 in the sameset, 6 in the subset. | ||
| + | |||
| + | **View 2**: list of all Proteins of the selected Protein Set. | ||
| + | |||
| + | **View 3**: list of all Peptides of the selected Protein | ||
| + | |||
| + | **View 4**: Protein Sequence of the previously selected Protein and Spectrum of the selected Peptide. | ||
| + | |||
| + | Note: Abbreviations used are listed [[how_to:studio:abbreviations|here]] | ||
| + | |||
| + | |||
| + | |||
| + | ===== PTM Protein Sites Window ===== | ||
| + | |||
| + | If you click on PTM Protein Sites sub-menu, you obtain this window: | ||
| + | |||
| + | {{:how_to:studio:ptmproteinsitewindow.png|}} | ||
| + | |||
| + | **View 1** (at the top): This view lists all Peptides/PTM. If a peptide contains multiple PTMs, it is displayed multiple times. | ||
| + | |||
| + | The user can click on "Hide Redundant Peptides" to hide the miss cleavage peptides with the same PTMs. | ||
| + | The non-redundant modifications are counted and displayed for each type of peptide. | ||
| + | |||
| + | The user can filter on PTM Modification. | ||
| + | |||
| + | In the following example, the user keeps only Oxidation on residue M. It is possible to specify no residue, to accept all, or to specify a list of residues. | ||
| + | |||
| + | {{:how_to:studio:ptmmodificationfilter.png|}} | ||
| + | |||
| + | **View 2** : All peptides of the selected Protein | ||
| + | |||
| + | **View 3** : Protein Sequence and selected peptide | ||
| + | |||
| + | ===== Adjacency Matrix Window ===== | ||
| + | |||
| + | If you click on Adjacency Matrix sub-menu, you obtain this window: | ||
| + | |||
| + | {{:how_to:studio:adjacencymatrixwindow.png|}} | ||
| - | Note : In the column Proteins, 8 (2,6) means that there are 8 proteins in the Protein Set. 2 in the sameset, 6 in the subset. | + | **View 1** : All the matrices. Each matrix correspond of a cluster composed of linked Proteins/Peptides. |
| - | **View 2** : list of all Proteins of the selected Protein Set. | + | **View 2** : The currently selected matrix. |
| - | **View 3** : list of all Peptides of the selected Protein | + | In the example, you can see that PIL1_YEAST and LSP1_YEAST share only one peptide. |
| - | **View 4** : Protein Sequence of the previously selected Protein and Spectrum of the selected Peptide. | ||
| - | Note : Abbreviations used are listed [[how_to:studio:abbreviations|here]] | ||