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how_to:studio:viewprotset [2014/06/20 15:57] 132.168.72.206 |
how_to:studio:viewprotset [2016/11/10 15:17] (current) 130.79.67.200 [PTM Protein Sites Window] |
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- | ====== Display PSM, Peptides or Protein Sets of an Identification Summary ====== | + | ====== Display MS Queries, PSM, Peptides, Protein Sets, PTM Protein Sites or Adjacency Matrices ====== |
- | ===== Functionnality Access ===== | + | ===== Functionality Access ===== |
To display data of an Identification Summary: | To display data of an Identification Summary: | ||
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- right click on an Identification Summary | - right click on an Identification Summary | ||
- | - click on the menu "Identification Summary >" and on the sub-menu "PSM", "Peptides" or "Protein Sets" | + | - click on the menu "Display Identification Summary >" and on the sub-menu "MSQueries", "PSM", "Peptides", "Protein Sets", "PTM Protein Sites" or "Adjacency Matrices" |
- | {{:how_to:studio:displayidspopupv3.png|}} | + | {{:how_to:studio:popupdisplayidentification.png|}} |
+ | |||
+ | |||
+ | |||
+ | ===== MSQueries Window ===== | ||
+ | |||
+ | If you click on MSQueries sub-menu, you obtain this window: | ||
+ | |||
+ | {{:how_to:studio:msqueryrsm.png|}} | ||
+ | |||
+ | Note: Abbreviations used are listed [[how_to:studio:abbreviations|here]] | ||
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{{:how_to:studio:displayidspsmdialogv3.png|}} | {{:how_to:studio:displayidspsmdialogv3.png|}} | ||
- | Note : Abbreviations used are listed [[how_to:studio:abbreviations|here]] | + | Note: Abbreviations used are listed [[how_to:studio:abbreviations|here]] |
===== Peptides Window ===== | ===== Peptides Window ===== | ||
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- | Upper View : list of all Peptides | + | **Upper View**: list of all Peptides |
- | Middle View : list of all Protein Sets containing the selected peptide. | + | **Middle View**: list of all Protein Sets containing the selected peptide. |
- | Bottom Left View : list of all Proteins of the selected Protein Set | + | **Bottom Left View**: list of all Proteins of the selected Protein Set |
- | Bottom Right View : list of all Peptides of the selected Protein | + | **Bottom Right View**: list of all Peptides of the selected Protein |
- | Note : Abbreviations used are listed [[how_to:studio:abbreviations|here]] | + | Note: Abbreviations used are listed [[how_to:studio:abbreviations|here]] |
===== Protein Sets Window ===== | ===== Protein Sets Window ===== | ||
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- | View 1 (at the top) : list of all Protein Sets | + | **View 1** (at the top): list of all Protein Sets |
+ | |||
+ | Note : In the column Proteins, 8 (2, 6) means that there are 8 proteins in the Protein Set. 2 in the sameset, 6 in the subset. | ||
+ | |||
+ | **View 2**: list of all Proteins of the selected Protein Set. | ||
+ | |||
+ | **View 3**: list of all Peptides of the selected Protein | ||
+ | |||
+ | **View 4**: Protein Sequence of the previously selected Protein and Spectrum of the selected Peptide. | ||
+ | |||
+ | Note: Abbreviations used are listed [[how_to:studio:abbreviations|here]] | ||
+ | |||
+ | |||
+ | |||
+ | ===== PTM Protein Sites Window ===== | ||
+ | |||
+ | If you click on PTM Protein Sites sub-menu, you obtain this window: | ||
+ | |||
+ | {{:how_to:studio:ptmproteinsitewindow.png|}} | ||
+ | |||
+ | **View 1** (at the top): This view lists all Peptides/PTM. If a peptide contains multiple PTMs, it is displayed multiple times. | ||
+ | |||
+ | The user can click on "Hide Redundant Peptides" to hide the miss cleavage peptides with the same PTMs. | ||
+ | The non-redundant modifications are counted and displayed for each type of peptide. | ||
+ | |||
+ | The user can filter on PTM Modification. | ||
+ | |||
+ | In the following example, the user keeps only Oxidation on residue M. It is possible to specify no residue, to accept all, or to specify a list of residues. | ||
+ | |||
+ | {{:how_to:studio:ptmmodificationfilter.png|}} | ||
+ | |||
+ | **View 2** : All peptides of the selected Protein | ||
+ | |||
+ | **View 3** : Protein Sequence and selected peptide | ||
+ | |||
+ | ===== Adjacency Matrix Window ===== | ||
+ | |||
+ | If you click on Adjacency Matrix sub-menu, you obtain this window: | ||
+ | |||
+ | {{:how_to:studio:adjacencymatrixwindow.png|}} | ||
- | Note : In the column Proteins, 4 (4,0) means that there are 4 proteins in the Protein Set. 4 in the sameset, 0 in the subset. | + | **View 1** : All the matrices. Each matrix correspond of a cluster composed of linked Proteins/Peptides. |
- | View 2 : list of all Proteins of the selected Protein Set. | + | **View 2** : The currently selected matrix. |
- | View 3 : list of all Peptides of the selected Protein | + | In the example, you can see that PIL1_YEAST and LSP1_YEAST share only one peptide. |
- | View 4 : Protein Sequence of the previously selected Protein and Spectrum of the selected Peptide. | ||
- | Note : Abbreviations used are listed [[how_to:studio:abbreviations|here]] |