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how_to:studio:rsvalidation [2013/10/07 09:47] 132.168.72.206 |
how_to:studio:rsvalidation [2016/07/08 08:23] (current) 132.168.72.225 [Validation Dialog] |
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====== How to validate a Search Result ====== | ====== How to validate a Search Result ====== | ||
- | See description of [[prolineconcepts:rsvalidation|validation algorithm here]]. | + | See description of [[prolineconcepts:rsvalidation|Validation Algorithm]]. |
- | To validate an Search Result: | + | |
+ | ===== Starting Validation ===== | ||
+ | |||
+ | {{:how_to:studio:validatepopupprolinev2.png|}} | ||
+ | |||
+ | To validate a Search Result: | ||
- Select one or multiple Search Results to validate | - Select one or multiple Search Results to validate | ||
Line 11: | Line 16: | ||
- Click on "Validate..." menu | - Click on "Validate..." menu | ||
- | {{:how_to:studio:validatepopupprolinev2.png|}} | ||
- | In the Validation Dialog, fill the different Parameters : | + | |
+ | ===== Validation Dialog ===== | ||
+ | |||
+ | {{:how_to:studio:validationdialogprolinev2.png|}} | ||
+ | |||
+ | In the Validation Dialog, fill the different Parameters (see [[prolineconcepts:rsvalidation|Validation description]]) : | ||
- you can add multiple PSM Prefilter Parameters ( Rank, Length, Score, e-Value, Identity p-Value, Homology p-Value) by selecting them in the combobox and clicking on Add Button '+' | - you can add multiple PSM Prefilter Parameters ( Rank, Length, Score, e-Value, Identity p-Value, Homology p-Value) by selecting them in the combobox and clicking on Add Button '+' | ||
- | - you can ensure a FDR on PSM which will reached according to the variable selected ( Score, e-Value, Identity p-Value, Homology p-Value ) | + | - you can ensure a FDR on PSM which will reached according to the variable selected ( Score, e-Value, Identity p-Value, Homology p-Value,... ) |
- | - you can add a Protein Set Prefilter on Specitifc Peptides. | + | - you can add a Protein Set Prefilter on Specific Peptides. |
- you can ensure a FDR on protein Sets. | - you can ensure a FDR on protein Sets. | ||
- | - you can set the choice for the Typical Protein of a Protein Set by using a match string with wildcards ( * or ? ) on Protein Accession or Protein Description. | + | - you can set the choice for the Typical Protein of a Protein Set by using a match string with wildcards ( * or ? ) on Protein Accession or Protein Description. (see [[how_to:studio:changetypicalprot|]]) |
- | Note : FDR can be used only for Search Results with Decoy Data. | + | Note: FDR can be used only for Search Results with Decoy Data. |
- | {{:how_to:studio:validationdialogprolinev2.png|}} | ||
- | Validating an identification can take some time. While it is not finished, the validated identifications are shown greyed with an hour glass over them. | ||
- | {{:how_to:studio:validationfinishedproline.png|}} | + | |
+ | ===== Validation Processing ===== | ||
+ | |||
+ | {{:how_to:studio:validationprocessingprolinev2.png|}} | ||
+ | |||
+ | Validating a Search Result can take some time. | ||
+ | While it is not finished, the Search Results are shown greyed with an hour glass over them. | ||
+ | The tasks are displayed as running in the "Tasks Log Dialog". | ||
+ | |||
+ | |||
+ | ===== Validation Done ===== | ||
+ | |||
+ | {{:how_to:studio:validationdoneprolinev2.png|}} | ||
+ | |||
+ | When the validation is finished, the icon becomes orange and blue. Orange part correspond to the Identification Summary. Blue is for the Search Result part. |