how_to:studio:rsvalidation
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how_to:studio:rsvalidation [2013/10/07 09:33] 132.168.72.206 |
how_to:studio:rsvalidation [2016/07/08 08:23] (current) 132.168.72.225 [Validation Dialog] |
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| ====== How to validate a Search Result ====== | ====== How to validate a Search Result ====== | ||
| - | See description of [[prolineconcepts:rsvalidation|validation algorithm here]]. | + | See description of [[prolineconcepts:rsvalidation|Validation Algorithm]]. |
| - | To validate an Search Result: | + | |
| + | ===== Starting Validation ===== | ||
| + | |||
| + | {{:how_to:studio:validatepopupprolinev2.png|}} | ||
| + | |||
| + | To validate a Search Result: | ||
| - Select one or multiple Search Results to validate | - Select one or multiple Search Results to validate | ||
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| - Click on "Validate..." menu | - Click on "Validate..." menu | ||
| - | {{:how_to:studio:validatepopupprolinev2.png|}} | ||
| - | In the Validation Dialog, fill the different Parameters : | ||
| - | - you can add multiple Peptides Prefilter Parameters ( Rank, Length, Score, e-Value, Identity p-Value, Homology p-Value) by selecting them in the combobox and clicking on Add Button '+' | + | ===== Validation Dialog ===== |
| + | |||
| + | {{:how_to:studio:validationdialogprolinev2.png|}} | ||
| + | |||
| + | In the Validation Dialog, fill the different Parameters (see [[prolineconcepts:rsvalidation|Validation description]]) : | ||
| + | |||
| + | - you can add multiple PSM Prefilter Parameters ( Rank, Length, Score, e-Value, Identity p-Value, Homology p-Value) by selecting them in the combobox and clicking on Add Button '+' | ||
| + | |||
| + | - you can ensure a FDR on PSM which will reached according to the variable selected ( Score, e-Value, Identity p-Value, Homology p-Value,... ) | ||
| + | |||
| + | - you can add a Protein Set Prefilter on Specific Peptides. | ||
| + | |||
| + | - you can ensure a FDR on protein Sets. | ||
| + | |||
| + | - you can set the choice for the Typical Protein of a Protein Set by using a match string with wildcards ( * or ? ) on Protein Accession or Protein Description. (see [[how_to:studio:changetypicalprot|]]) | ||
| + | |||
| + | Note: FDR can be used only for Search Results with Decoy Data. | ||
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| + | ===== Validation Processing ===== | ||
| - | - you can ensure a FDR on peptides which will reached according to the variable selected ( Score, e-Value, Identity p-Value, Homology p-Value ) | + | {{:how_to:studio:validationprocessingprolinev2.png|}} |
| - | - you can select the FDR estimator | + | Validating a Search Result can take some time. |
| + | While it is not finished, the Search Results are shown greyed with an hour glass over them. | ||
| + | The tasks are displayed as running in the "Tasks Log Dialog". | ||
| - | - you can ensure a FDR on proteins | ||
| - | FDR can be used only for Identification with Decoy Data. | + | ===== Validation Done ===== |
| - | {{:how_to:studio:validationdialogproline.png|}} | + | {{:how_to:studio:validationdoneprolinev2.png|}} |
| - | Validating an identification can take some time. While it is not finished, the validated identifications are shown greyed with an hour glass over them. | + | When the validation is finished, the icon becomes orange and blue. Orange part correspond to the Identification Summary. Blue is for the Search Result part. |
| - | {{:how_to:studio:validationfinishedproline.png|}} | ||
how_to/studio/rsvalidation.1381131186.txt.gz · Last modified: 2013/10/07 09:33 by 132.168.72.206
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