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--- how_to:studio:rsvalidation [2013/03/01 15:54]
132.168.72.130
+++ how_to:studio:rsvalidation [2016/07/08 08:23] (current)
132.168.72.225 [Validation Dialog]
@@ Line 1: / Line 1: @@
-====== How to validate an Identification ======
+====== How to validate a Search Result ======
-See description of [[prolineconcepts:rsvalidation|validation algorithm here]].
+See description of [[prolineconcepts:rsvalidation|Validation Algorithm]].
-To validate an Identification :
-- Select one or multiple identifications to valid
+===== Starting Validation =====
+{{:how_to:studio:validatepopupprolinev2.png|}}
+To validate a Search Result:
+- Select one or multiple Search Results to validate
 - Right Click to display the popup
@@ Line 11: / Line 16: @@
 - Click on "Validate..." menu
-{{:how_to:studio:validatepopupproline.png|}}
+===== Validation Dialog =====
+{{:how_to:studio:validationdialogprolinev2.png|}}
+In the Validation Dialog, fill the different Parameters (see [[prolineconcepts:rsvalidation|Validation description]]) :
+- you can add multiple PSM Prefilter Parameters ( Rank, Length, Score, e-Value, Identity p-Value, Homology p-Value) by selecting them in the combobox and clicking on Add Button '+'
+- you can ensure a FDR on PSM which will reached according to the variable selected ( Score, e-Value, Identity p-Value, Homology p-Value,... )
+- you can add a Protein Set Prefilter on Specific Peptides.
+- you can ensure a FDR on protein Sets.
+- you can set the choice for the Typical Protein of a Protein Set by using a match string with wildcards ( * or ? ) on Protein Accession or Protein Description. (see [[how_to:studio:changetypicalprot|]])
+Note: FDR can be used only for Search Results with Decoy Data.
+===== Validation Processing =====
+{{:how_to:studio:validationprocessingprolinev2.png|}}
+Validating a Search Result can take some time.
+While it is not finished, the Search Results are shown greyed with an hour glass over them.
+The tasks are displayed as running in the "Tasks Log Dialog".
+===== Validation Done =====
+{{:how_to:studio:validationdoneprolinev2.png|}}
+When the validation is finished, the icon becomes orange and blue. Orange part correspond to the Identification Summary. Blue is for the Search Result part.

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