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how_to:studio:rsvalidation [2013/02/25 07:48]
132.168.72.131 créée
how_to:studio:rsvalidation [2016/07/08 08:23] (current)
132.168.72.225 [Validation Dialog]
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-====== How to validate a ResultSet  ​======+====== How to validate a Search Result ​======
  
-See [[prolineconcepts:​rsvalidation|validation algorithm here]].+See description of [[prolineconcepts:​rsvalidation|Validation Algorithm]].
  
  
-Validation ​can be done on 1 or more identification result. Once desired identification result are selected, right-click and select Validation..... ​+===== Starting ​Validation ​=====
  
-ScreenShots  +{{:​how_to:​studio:​validatepopupprolinev2.png|}}
-  ..+
  
-Result ​View  +To validate a Search ​Result:
-   ...+
  
 +- Select one or multiple Search Results to validate
 +
 +- Right Click to display the popup
 +
 +- Click on "​Validate..."​ menu
 +
 +
 +
 +
 +===== Validation Dialog =====
 +
 +{{:​how_to:​studio:​validationdialogprolinev2.png|}}
 +
 +In the Validation Dialog, fill the different Parameters (see [[prolineconcepts:​rsvalidation|Validation description]]) :
 +
 +- you can add multiple PSM Prefilter Parameters ( Rank, Length, Score, e-Value, Identity p-Value, Homology p-Value) by selecting them in the combobox and clicking on Add Button '​+'​
 +
 +- you can ensure a FDR on PSM which will reached according to the variable selected ( Score, e-Value, Identity p-Value, Homology p-Value,... )
 +
 +- you can add a Protein Set Prefilter on Specific Peptides.
 +
 +- you can ensure a FDR on protein Sets.
 +
 +- you can set the choice for the Typical Protein of a Protein Set by using a match string with wildcards ( * or ? ) on Protein Accession or Protein Description. (see [[how_to:​studio:​changetypicalprot|]])
 +
 +Note: FDR can be used only for Search Results with Decoy Data.
 +
 +
 +
 +
 +===== Validation Processing =====
 +
 +{{:​how_to:​studio:​validationprocessingprolinev2.png|}}
 +
 +Validating a Search Result can take some time.
 +While it is not finished, the Search Results are shown greyed with an hour glass over them.
 +The tasks are displayed as running in the "Tasks Log Dialog"​.
 +
 +
 +===== Validation Done =====
 +
 +{{:​how_to:​studio:​validationdoneprolinev2.png|}}
 +
 +When the validation is finished, the icon becomes orange and blue. Orange part correspond to the Identification Summary. Blue is for the Search Result part.
  
how_to/studio/rsvalidation.1361774930.txt.gz · Last modified: 2013/02/25 07:48 by 132.168.72.131