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how_to:studio:rsvalidation [2013/02/25 07:48] 132.168.72.131 créée |
how_to:studio:rsvalidation [2016/07/08 08:23] (current) 132.168.72.225 [Validation Dialog] |
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- | ====== How to validate a ResultSet ====== | + | ====== How to validate a Search Result ====== |
- | See [[prolineconcepts:rsvalidation|validation algorithm here]]. | + | See description of [[prolineconcepts:rsvalidation|Validation Algorithm]]. |
- | Validation can be done on 1 or more identification result. Once desired identification result are selected, right-click and select Validation..... | + | ===== Starting Validation ===== |
- | ScreenShots | + | {{:how_to:studio:validatepopupprolinev2.png|}} |
- | ... | + | |
- | Result View | + | To validate a Search Result: |
- | ... | + | |
+ | - Select one or multiple Search Results to validate | ||
+ | |||
+ | - Right Click to display the popup | ||
+ | |||
+ | - Click on "Validate..." menu | ||
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+ | |||
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+ | |||
+ | ===== Validation Dialog ===== | ||
+ | |||
+ | {{:how_to:studio:validationdialogprolinev2.png|}} | ||
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+ | In the Validation Dialog, fill the different Parameters (see [[prolineconcepts:rsvalidation|Validation description]]) : | ||
+ | |||
+ | - you can add multiple PSM Prefilter Parameters ( Rank, Length, Score, e-Value, Identity p-Value, Homology p-Value) by selecting them in the combobox and clicking on Add Button '+' | ||
+ | |||
+ | - you can ensure a FDR on PSM which will reached according to the variable selected ( Score, e-Value, Identity p-Value, Homology p-Value,... ) | ||
+ | |||
+ | - you can add a Protein Set Prefilter on Specific Peptides. | ||
+ | |||
+ | - you can ensure a FDR on protein Sets. | ||
+ | |||
+ | - you can set the choice for the Typical Protein of a Protein Set by using a match string with wildcards ( * or ? ) on Protein Accession or Protein Description. (see [[how_to:studio:changetypicalprot|]]) | ||
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+ | Note: FDR can be used only for Search Results with Decoy Data. | ||
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+ | |||
+ | |||
+ | |||
+ | ===== Validation Processing ===== | ||
+ | |||
+ | {{:how_to:studio:validationprocessingprolinev2.png|}} | ||
+ | |||
+ | Validating a Search Result can take some time. | ||
+ | While it is not finished, the Search Results are shown greyed with an hour glass over them. | ||
+ | The tasks are displayed as running in the "Tasks Log Dialog". | ||
+ | |||
+ | |||
+ | ===== Validation Done ===== | ||
+ | |||
+ | {{:how_to:studio:validationdoneprolinev2.png|}} | ||
+ | |||
+ | When the validation is finished, the icon becomes orange and blue. Orange part correspond to the Identification Summary. Blue is for the Search Result part. | ||