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how_to:studio:datamixer [2016/02/08 14:41]
132.168.72.206
how_to:studio:datamixer [2016/07/04 11:50] (current)
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 ===== Available Functions ===== ===== Available Functions =====
 +
 +==== compute FDR Function ====
 +
 +This function is used by ProStar Macro to compute the FDR.
 +
 +More information:​
 +http://​bioconductor.org/​packages/​release/​bioc/​vignettes/​Prostar/​inst/​doc/​Prostar_UserManual.pdf
  
 ==== join Function ==== ==== join Function ====
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 When a key value is not found in one of the data source table, the line is displayed as empty. For numerical values a difference is done and for string values, the '<>'​ symbol is displayed when values are different. When a key value is not found in one of the data source table, the line is displayed as empty. For numerical values a difference is done and for string values, the '<>'​ symbol is displayed when values are different.
  
-==== pvalue and ttd Functions ​====+==== adjustp Function / calibration Plot ====
  
-pvalue and ttd functions are useful ​for XIC Quantitations+Calibration Plot for Proteomics is discribed here : https://​cran.r-project.org/​web/​packages/​cp4p/​index.html
  
 ==== bbinomial Function ==== ==== bbinomial Function ====
Line 53: Line 60:
 bbinomial function is useful for Spectral Count Quantitations bbinomial function is useful for Spectral Count Quantitations
  
 +==== diff analysis Function ====
 +
 +This function is used by ProStar Macro.
 +Two tests are available : Welch t-test and Limma t-test.
 +
 +More information:​
 +http://​bioconductor.org/​packages/​release/​bioc/​vignettes/​Prostar/​inst/​doc/​Prostar_UserManual.pdf
 +
 +==== filter Function ====
 +
 +filter function let the user filter some rows of a matrix according to settings on columns
 +
 +==== import CSV/TSV ====
 +
 +This module lets you import data from a CSV or TSV file. Then you can do calculations and display these data directly in Proline Studio.
 +
 +==== missing values filter Function ====
 +
 +This function is used by ProStar Macro to remove rows with too many missing quantitative values.
 +
 +The available missing values algorithm are:
 +  * Whole Groups: The lines (across all groups) in the quantitative dataset which contain less non-missing value than a user-defined threshold are deleted.
 +  * For every group: The lines for which each condition contain less non-missing value than a user-defined threshold are deleted.
 +  * At least one group: The lines for which at least one condition contain less non-missing value than a user-defined threshold are deleted.
 +
 +==== missing values imputation Function ====
 +
 +This function is used by ProStar Macro to imput missing values.
 +
 +More information:​
 +http://​bioconductor.org/​packages/​release/​bioc/​vignettes/​Prostar/​inst/​doc/​Prostar_UserManual.pdf
 +
 +
 +==== normalize Function ====
 +This function is used by ProStar Macro to normalize quantitative values.
 +
 +More information on algorithms : http://​bioconductor.org/​packages/​release/​bioc/​vignettes/​Prostar/​inst/​doc/​Prostar_UserManual.pdf
 +
 +
 +==== pvalue and ttd Functions ====
 +
 +pvalue and ttd functions are useful for XIC Quantitations
 +
 +
 +===== Prostar Macro =====
 +
 +{{:​how_to:​studio:​prostarcheminement.png?​800|}}
 +
 +1 : Add XIC Data to Data Analyzer from the Protein Set View.
 +
 +2 : Add Prostar Macro by a drag and drop and link XIC Data to the Macro
 +
 +3 : Process the Graph by clicking on the button
 +
 +During the process, the Data Analyzer will ask you settings for each function.
 +
 +4 : Filter is needed only if you want to remove contaminants. Settings can be
 +validated with no parameters if you don't need it.
 +
 +5 : Log is needed to log abundances (Data from Proline). For Data coming from Max Quant, data
 +is already logged.
 +
 +6 to 12 : follow the settings asked ( you can find some help in Prostar documentation,​ or information in corresponding functions.)
 +
 +During the process, results will be automatically displayed : calibration,​ scatter plot and FDR Result.
 +If you want to look at other results, right click on a function and select "​Display in New Window"​
  
 +Prostar User Manual : http://​bioconductor.org/​packages/​release/​bioc/​vignettes/​Prostar/​inst/​doc/​Prostar_UserManual.pdf
  
 +Prostar Tutorial : 
 +http://​bioconductor.org/​packages/​release/​bioc/​vignettes/​Prostar/​inst/​doc/​Prostar_Tutorial.pdf
 + 
  
how_to/studio/datamixer.1454938876.txt.gz · Last modified: 2016/02/08 14:41 by 132.168.72.206