userguide:contextcomparisons
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userguide:contextcomparisons [2011/02/01 14:41] 132.168.74.230 |
userguide:contextcomparisons [2012/02/28 11:18] 132.168.73.124 |
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| {{:diceCoef.png?102x50}} | {{:diceCoef.png?102x50}} | ||
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| + | ===== Details about comparison results ===== | ||
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| + | When the comparison algorithm execution is finished, the comparison result is displayed in a tab.\\ | ||
| + | The most left column ("Union reference") displays the reference protein groups (master protein accession) from the union parent context (it may be a virtual context).\\ | ||
| + | For each protein group of the Union Reference context, the table displays the most "similar" protein group in each compared contexts.\\ | ||
| + | For each compared context a default set of properties are displayed. You can change them by selecting/deselecting items in the droplist (right side). | ||
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| + | {{:userguide:comparevirtualconcept_3.png|}} | ||
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| + | ==== Comparison result view ==== | ||
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| + | * __ProtSim__: similarity on the **samesets of proteins** of the two protein groups\\ | ||
| + | * __AllProtSim__: similarity on the **samesets and subsets of proteins** of the two protein groups\\ | ||
| + | * __PepSim__: similarity on **peptides** of the two protein groups | ||
| + | * __isSameTypical__: indicates if the **typical protein is the same** between the two protein groups | ||
| + | * __Typical__: the **accession of the master** protein group | ||
| + | * :!: **NEW** __refLoc__: **possible values are [TYPICAL], [SAMESET], [SUBSET] or []**. This indicates where the reference protein accession is seen in the compared protein group. | ||
| + | * [TYPICAL] means the reference accession is seen in the compared protein group as the typical protein | ||
| + | * [SAMESET] means the reference accession is seen in the compared protein group as a sameset protein | ||
| + | * [SUBSET] means the reference accession is seen in the compared protein group as a subset protein | ||
| + | * [] means the reference accession is not seen in the compared protein group. In this case, ProtSim = 0 and pepSim > 0. | ||
| + | * :!: **NEW** __refSC__: the **spectral count of the reference protein accession** in the compared protein grouped. :!: **No need to run previously the spectral count on the context**. | ||
| + | * :!: **NEW** __refSpeSC__: the **specific (proteotypic) spectral count of the reference protein** in the compared protein group. :!: **No need to run previously the spectral count on the context**. | ||
| + | * __Score__: **score** of the compared protein group | ||
| + | * __#Pep__: **number of peptides** of the compared protein group | ||
| + | * __#AllProt__: **number of proteins** in the compared protein group. | ||
| + | * 'X(Y,Z)' means a total of X proteins for the whole protein group (X=Y+Z) with Y sameset proteins and Z subset proteins | ||
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| ===== Compare several contexts to their union ===== | ===== Compare several contexts to their union ===== | ||
| - | The purpose is to select contexts to compare, then to compare them to their union, i.e. a parent context, which contains selected children contexts, and for which peptide/protein grouping has been executed.\\ | + | Main steps realized in the comparison process: |
| + | - Retrieve selected contexts to compare | ||
| + | - Know if they are all the children of an existing parent context. If not, create a virtual union parent context (see details in following section) that groups selected contexts and run peptide/protein grouping | ||
| + | - Compare selected contexts to their union (virtual or existing parent) | ||
| ==== Is there a need to create a "virtual" union parent context ? ==== | ==== Is there a need to create a "virtual" union parent context ? ==== | ||
| - | There are 2 possibilities, either the parent union already exists or the parent union needs to be created.\\ | + | There are 2 situations, either the parent union already exists or the parent union needs to be created. In the image below: |
| - | In the image below: | + | * A *virtual* union parent context must be created in **case 1** (the virtual context has no persistence in MSI database, it is only created in-memory for the process) |
| - | * the union parent must be created in **case 1**: it is called //**virtual**// as it is only created in-memory for the process | + | * The union parent context already exists in **case 2** |
| - | * the union parent already exists in **case 2** | + | |
| {{:userguide:comparevirtualconcept_1.png|}} | {{:userguide:comparevirtualconcept_1.png|}} | ||
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| ^ AllProtSimilarity | - | - | | ^ AllProtSimilarity | - | - | | ||
| - | ===== Details about comparison results ===== | + | ==== Why/What means High/Low peptide/protein similarities ==== |
| - | When the comparison algorithm execution has finished, a window displays on the screen.\\ | + | {{:groupingsamesetsubset.png |How to obtain a low Protein similarity but with a high Peptide similarity}} When comparing two protein groups using "Protein" and "Peptide" similarity criteria, you can get four main situations: |
| - | The most left column ("Union reference") displays protein groups (master protein accession) from the (virtual) union parent context.\\ | + | |
| - | For each protein group of the Union Reference context, the table displays the most "similar" protein group in each compared contexts.\\ | + | |
| - | For each compared context a default set of properties are displayed. You can change them by selecting/deselecting items in the droplist (right side). | + | |
| - | + | ||
| - | {{:userguide:comparevirtualconcept_3.png|}} | + | |
| - | + | ||
| - | + | ||
| - | {{:groupingsamesetsubset.png |How to obtain a low Protein similarity but with a high Peptide similarity}} Protein group properties: | + | |
| - | * "ProtSim": similarity on the **samesets of proteins** of the two protein groups\\ | + | |
| - | * "AllProtSim": similarity on the **samesets and subsets of proteins** of the two protein groups\\ | + | |
| - | * "PepSim": similarity on **peptides** of the two protein groups | + | |
| - | * "isSameTypical": indicates if the typical protein is the same between the two protein groups | + | |
| - | * "Typical": the accession of the master protein group | + | |
| - | * "Score": score for the protein group | + | |
| - | * "#Pep": number of peptides for the protein group | + | |
| - | * "#AllProt": number of proteins in the protein group. 'X(Y,Z)' means a total of X proteins for the whole protein group (X=Y+Z) with Y sameset proteins and Z subset proteins | + | |
| - | * If Spectral Count algorithm is run previously, Spectral Count values will be available as well | + | |
| - | + | ||
| - | When comparing two protein groups using "Protein" and "Peptide" similarity criteria, you can get four main situations: | + | |
| | ^ Low Protein similarity ^ High Protein similarity ^ | | ^ Low Protein similarity ^ High Protein similarity ^ | ||
| ^ Low Peptide similarity | Proteins groups are **not** alike | We have identified the same proteins but with different peptides (rare)| | ^ Low Peptide similarity | Proteins groups are **not** alike | We have identified the same proteins but with different peptides (rare)| | ||
| ^ High Peptide similarity | See image (on the left) to have an example of how to get this situation. It's usefull to check the "All Protein" similarity to have an information on proteins in subset| protein group are alike| | ^ High Peptide similarity | See image (on the left) to have an example of how to get this situation. It's usefull to check the "All Protein" similarity to have an information on proteins in subset| protein group are alike| | ||
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userguide/contextcomparisons.txt · Last modified: 2012/02/28 11:18 by 132.168.73.124
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