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| - | ====== Protein grouping ====== | ||
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| - | ===== Algorithm ===== | ||
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| - | ==== Step 1 - Peptide grouping ==== | ||
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| - | Peptides from different identifications - attached directly or indirectly to a context - are grouped.\\ | ||
| - | Peptides must have same sequence and same experimental mass to be grouped.\\ | ||
| - | Peptide grouping results in **new** peptides attached to the parent context and having child peptides | ||
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| - | {{proteinGrouping:GroupingStep1.png?248x148}} | ||
| - | ==== Step 2 - Protein grouping ==== | ||
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| - | Peptides from a same protein or a same protein set are grouped.\\ | ||
| - | Protein grouping results in **new** groups of proteins and peptides, attached to the parent context. | ||
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| - | {{proteinGrouping:GroupingStep2.png?271x186}} | ||
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| - | ===== GUI - Execute protein grouping algorithm ===== | ||
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| - | - Right-click on the context node of your choice in the MSIExplorer view to display context sensitive menu and select "Algorithms->Group proteins..." {{ :proteingrouping:proteingroupingaction.png?300|Execute protein grouping}} | ||
| - | - Change parameters for protein grouping if needed. Validate. | ||
| - | - When grouping is finished, the color of the context name change to red in the MSIExplorer view. | ||
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| - | {{:proteingrouping:proteingroupingparam.png?350|Parameters for protein grouping}} | ||
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