README VM VIRFAM username : meije password : Meije7474 To import a file, use scp or a USB stick - to activate a USB port in the VM: start the VM plug the USB stick in a USB port right click on the USB icon in the lower right corner of the VM check the box corresponding to the USB you want to use in the VM /!\ unmount the USB stick before powering off the VM /!\ when the USB stick is available on the VM, it's no more available for the host - to start virfam : README.txt in the VM : ~/package-virfam/README.txt Please note that the virfam.cfg file should be copied into the running directory - VM settings : Default settings of the VM : 4 CPU, 8GB RAM and 50GB disk memory If the computer cannot support these settings, you can reduce CPU and RAM but not the disk memory. Effect of the number of CPU on execution time : (8GB RAM ; 50GB disk memory ; test on a 12 sequences file) nb of CPU | virfam execution time 1 | 25 minutes 2 | 13 minutes 3 | 10 min 30 S 4 | 9 minutes 5 | 8 min 30 s 6 | 7 min 45 s Effect of the RAM on execution time : (4 CPU ; 50GB disk memory ; test on a 12 sequences file) RAM | virfam execution time 4GB | 25 minutes 5GB | 12 minutes 6GB | 9 minutes 8GB | 8 min 45 s Multithreading blastpgp : Effect of the multithreading of blastpgp on execution time : (4 CPU ; 8 GB RAM ; 50 GB disk memory ; test on a 12 sequences file) threads | execution time 1 | 25 minutes 2 | 13 minutes 3 | 10 minutes 4 | 8 min 45 s modification of the number of threads : If the CPU number has to be set below 4, change also the ncpu_blast parameter in the virfam.cfg file and set it to the same value as the CPU number. see configuration file 'virfam.cfg' line 42 ncpu_blast = 4