firststeps
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| ====== Getting Started ====== | ====== Getting Started ====== | ||
| - | This tutorial will show you how to validate a Mascot identification result with IRMa. A sample Mascot result file is available in the [[ftp://ftp.cea.fr/pub/edyp/irma|download section]] : ''result_sample.dat''. But the same operation could be executed with any Mascot MS/MS Ion Search result. | + | This tutorial will show you how to validate a Mascot identification result with IRMa. A sample Mascot result file is available in the [[ftp://ftp.cea.fr/pub/edyp/irma|download section]] : ''result_sample.dat''. But the same operation could be executed with any Mascot MS/MS Ion Search result. |
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| + | **Note**: The ''.dat'' file should not be stored in a directory containing accent or blank. | ||
| ===== Step 1 : Run IRMa ===== | ===== Step 1 : Run IRMa ===== | ||
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| ===== Step 2 : Open and Parse a result file ===== | ===== Step 2 : Open and Parse a result file ===== | ||
| + | **Note**: The ''.dat'' file should not be stored in a directory containing accent or blank. | ||
| + | |||
| Select Menu File --> Open --> Mascot Identification. In the //open dialog// select the ''result_sample.dat'' file. | Select Menu File --> Open --> Mascot Identification. In the //open dialog// select the ''result_sample.dat'' file. | ||
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| {{ filter_settings.png?300 | Filter settings}} | {{ filter_settings.png?300 | Filter settings}} | ||
| - | ===== Step 4 : Browse result ===== | + | ===== Step 4 : Result validation ===== |
| From Protein Centric Navigation Panel, you can select any identified protein hit to view its property (see User Guide Description...FIXME) | From Protein Centric Navigation Panel, you can select any identified protein hit to view its property (see User Guide Description...FIXME) | ||
| - | |||
| - | ===== Step 5 : Result validation ===== | ||
| They can be identified in the Protein Centric Navigation Panel, they are grayed | They can be identified in the Protein Centric Navigation Panel, they are grayed | ||
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| + | {{ hit_search.png?500 | Hit search}} | ||
| For example, | For example, | ||
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| It is also possible to delete all protein hits with no relevant peptides by clicking on <IMG_ALL_DEL> in general toolbox. | It is also possible to delete all protein hits with no relevant peptides by clicking on <IMG_ALL_DEL> in general toolbox. | ||
| - | ===== Step 6 : Export validated result ===== | + | ===== Step 5 : Export validated result ===== |
| - | + | ||
| + | Once validated, the identification result can be exported into various formats. Click File -->Export --> Identification result --> to Excel. Choose the name of the file that will be generated and click Save | ||
firststeps.1236264889.txt.gz · Last modified: 2009/03/05 15:54 by 132.168.73.124
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